abstract：:Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...

journal_title：Canadian journal of microbiology

authors： Fliermans CB,Hazen TC

更新日期：1980-02-01 00:00:00

abstract：:Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose,...

journal_title：Canadian journal of microbiology

authors： Sprott GD,McKellar RC

更新日期：1980-02-01 00:00:00

abstract：:The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...

journal_title：Canadian journal of microbiology

authors： Bannatyne RM,Cheung R

更新日期：1980-02-01 00:00:00

abstract：:A microcinematographic analysis of the behaviour and movements of cells and cell masses in mated cultures (NC4 X VI2) of Dictyostelium discoideum indicates that a chemotactic process directs cell aggregation during macrocyst development. Zygote giant cells form before aggregation begins and act as the aggregation cent...

journal_title：Canadian journal of microbiology

authors： O'Day DH

更新日期：1979-12-01 00:00:00

abstract：:During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...

journal_title：Canadian journal of microbiology

authors： McCready RG,Krouse HR

更新日期：1979-12-01 00:00:00

abstract：:A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...

journal_title：Canadian journal of microbiology

authors： Saddler JN,Khan AW

更新日期：1979-12-01 00:00:00

abstract：:Mixtures of anionic HgCl3-/HgCl4(2)-complexes were less toxic to terrestrial bacteria (Erwinia herbicola, Agrobacterium tumefaciens), to marine bacteria (Acinetobacter sp., Aeromonas sp.), and to bacteriophages (phi 11 M 15 of Staphylococcus aureus and P1 of Escherichia coli) than were equivalent concentrations of Hg ...

journal_title：Canadian journal of microbiology

authors： Babich H,Stotzky G

更新日期：1979-11-01 00:00:00

abstract：:Two strains of Bacillus sphaericus. SSII-1 and 1593, were bioassayed for toxic activity against second-instar larvae of the mosquito Culex pipiens quinquefasciatus. It was found that strain 1593 developed a level of toxicity 3000 times that of strain SSII-1. Although the toxic activity of B. sphaericus SSII-1 was rela...

journal_title：Canadian journal of microbiology

authors： Myers P,Yousten AA,Davidson EW

更新日期：1979-11-01 00:00:00

abstract：:In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...

journal_title：Canadian journal of microbiology

authors： Ishiguro EE

更新日期：1979-10-01 00:00:00

abstract：:Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...

journal_title：Canadian journal of microbiology

authors： Stevenson RM

更新日期：1979-10-01 00:00:00

abstract：:There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...

journal_title：Canadian journal of microbiology

authors： Sherry JP,Kuchma SR,Dutka BJ

更新日期：1979-09-01 00:00:00

abstract：:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...

journal_title：Canadian journal of microbiology

authors： Ogg JE,Lee SY,Ogg BJ

更新日期：1979-09-01 00:00:00

abstract：:Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...

journal_title：Canadian journal of microbiology

authors： Compton SW,Mayo JA,Ehrlich M,Ackermann HW,Tremblay L,Cords CE,Scaletti JV

更新日期：1979-09-01 00:00:00

abstract：:Three distinct bacterial populations have been defined in the bovine rumen: the rumen fluid population; the population associated with food particles; and the population adherent to the rumen epithelium. Alkaline phosphatase activity has been reported in cells of the first two populations and here we report that assay...

journal_title：Canadian journal of microbiology

authors： Fay JP,Cheng KJ,Costerton JW

更新日期：1979-08-01 00:00:00

abstract：:Among nine eluents tested, 0.5% (w/v) isoelectric casein at pH 9.0 and 0.5% (w/v) non-fat dry milk (pH 9.0) were the most efficient in eluting poliovirus type 1 (Sabin) from Eustis fine sand. However, no significant difference was found between the overall (elution followed by concentration) virus recoveries by non-fa...

journal_title：Canadian journal of microbiology

authors： Bitton G,Charles MJ,Farrah SR

更新日期：1979-08-01 00:00:00

abstract：:Seventeen of 22 group A meningococcal strains were separated into four serotypes by a microbactericidal assay using group A antisera. In addition, 6 of 19 of these strains were killed by a group B antiserum and similar cidal patterns were obtained with a group C antiserum. There was little correlation between type and...

journal_title：Canadian journal of microbiology

authors： Quan AL,Sippel JE

更新日期：1979-08-01 00:00:00

abstract：:The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...

journal_title：Canadian journal of microbiology

authors： Mehta RJ,Nash CH

更新日期：1979-07-01 00:00:00

abstract：:The antiserum agar method (ASA), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of Neisseria meningitidis. Four hundred meningococcal stains were serogrouped by ASA employing horse antise...

journal_title：Canadian journal of microbiology

authors： Ashton FE,Ryan A,Diena BB,Frasch CE

更新日期：1979-06-01 00:00:00

abstract：:Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be compos...

journal_title：Canadian journal of microbiology

authors： Casida LE Jr,Liu KC

更新日期：1979-06-01 00:00:00

abstract：:From electron-microscopical observations, a decreased metabolic activity in 3-day-old Candida albicans chlamydospores was suggested, and progressive deterioration in chlamydospores aged 2-8 months was shown. Oxygen utilization by chlamydospore-pseudomycelium (CSP) preparations was less than that by yeast, while 3-day-...

journal_title：Canadian journal of microbiology

authors： Miller SE,Finnerty WR

更新日期：1979-06-01 00:00:00

abstract：:Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...

journal_title：Canadian journal of microbiology

authors： MacInnes JI,Trust TJ,Crosa JH

更新日期：1979-05-01 00:00:00

abstract：:A defined medium (CDM) is described which supported growth and sporulation of type E strains of Clostridium botulinum, but not sporulation of other serotypes of C. botulinum or C. sporogenes. As compared to growth in complex medium, spore outgrowth was delayed and both the growth rate and the cell yield was reduced. H...

journal_title：Canadian journal of microbiology

authors： Hawirko RZ,Naccarato CA,Lee RP,Maeba PY

更新日期：1979-04-01 00:00:00

abstract：:The induction of nitrogenase (C2H2) activity in asymbiotically cultured Rhizobium sp. 32H1 was found to be associated with morphological changes in the cells which were more pronounced than those seen in bacteroids. Polyphosphate granules were found in both bacteroids and cultured cells, but poly-beta-hydroxybutyrate ...

journal_title：Canadian journal of microbiology

authors： van Brussel AA,Costerton JW,Child JJ

更新日期：1979-03-01 00:00:00

abstract：:Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...

journal_title：Canadian journal of microbiology

authors： Middlebrook JL,Dorland RB

更新日期：1979-03-01 00:00:00

abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...

journal_title：Canadian journal of microbiology

authors： Kogure K,Simidu U,Taga N

更新日期：1979-03-01 00:00:00

abstract：:Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as ca...

journal_title：Canadian journal of microbiology

authors： Liao CH,Heberlein GT

更新日期：1979-02-01 00:00:00

abstract：:Candida tropicalis synthesizes a hydroxylase (3 to 5 nmol of product formed per minute per milligram of protein) and a cytochrome P-450 (0.10 to 0.13 nmol per milligram of protein) during growth on n-tetradecane. A three- to four-fold increase in the level of NADPH cytochrome c reductase is also observed in those cell...

journal_title：Canadian journal of microbiology

authors： Gilewicz M,Zacek M,Bertrand JC,Azoulay E

更新日期：1979-02-01 00:00:00

abstract：:Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...

journal_title：Canadian journal of microbiology

authors： Nelson LM,Parkinson D

更新日期：1978-12-01 00:00:00

abstract：:A purified lipopolysaccharide (LPS) isolated from the rickettsial agent of Q fever, Coxiella burnetii phase 1, possesses some of the in vivo and in vitro biological properties previously only associated with bacterial endotoxins. The Coxiella LPS is mitogenic for guinea pig leukocytes, induces non-specific resistance ...

journal_title：Canadian journal of microbiology

authors： Paquet A Jr,Rael ED,Klassen D,Martinez I,Baca OG

更新日期：1978-12-01 00:00:00

abstract：:Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

journal_title：Canadian journal of microbiology

authors： Nyhlén L,Unestam T

更新日期：1978-11-01 00:00:00

abstract：:Fungal cultures were grown by a new microslide method and by the traditional slide-culture method. Sporulation results were similar by both methods. The microslide method has the advantages of increased safety to the user and ease of storage. ...

journal_title：Canadian journal of microbiology

authors： Hofherr L

更新日期：1978-10-01 00:00:00

abstract：:Growth rates and cell yields of Mycobacterium convolutum strain R22 grown on a wide range of both odd- and even-numbered carbon solid n-alkanes decreased as the substrate carbon number increased. Cellular lipid was 2.5 times higher following growth on the hydrocarbon substrates. The amount of polar lipid was found to ...

journal_title：Canadian journal of microbiology

authors： Hallas LE,Vestal JR

更新日期：1978-10-01 00:00:00

abstract：:A moderately halophilic Acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. Maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2M NaCl or 1M KCl. Two kinds of amylase, amylases I and II were purified from the culture fil...

journal_title：Canadian journal of microbiology

authors： Onishi H,Hidaka O

更新日期：1978-09-01 00:00:00

abstract：:Histological examination of oesophagus and stomach specimens from 28 guinea pigs (Cavia porcellus) revealed microbes associated with the epithelial surface of these organs. Layers of Gram-positive cocci were associated with oesophageal tissue in all the animals. Yeasts (Torulop-sid pintolopesii) were associated with s...

journal_title：Canadian journal of microbiology

authors： Yoon CS,Tannock GW

更新日期：1978-09-01 00:00:00

abstract：:This study was undertaken to determine if direct cytotoxicity (DC) against herpes simplex virus infected cells, perhaps mediated by T cells, could be demonstrated in individuals subject to recurrent herpes labialis. The mononuclear cells from 7 out of 17 individuals with recurrent herpes expressed DC whereas no DC was...

journal_title：Canadian journal of microbiology

authors： Fujimiya Y,Babiuk LA,Rouse BT

更新日期：1978-09-01 00:00:00

abstract：:Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...

journal_title：Canadian journal of microbiology

authors： Knoop FC

更新日期：1978-08-01 00:00:00

abstract：:The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...

journal_title：Canadian journal of microbiology

authors： Blakley ER

更新日期：1978-07-01 00:00:00

abstract：:Schizophyllum commune Fr. was shown, by light, scanning, and transmission electron microscopy, to be a destructive mycoparasite on several phytopathogenic and nematode-trapping fungi. The hyphae of S. commune coiled around host hyphae and fruiting structures and penetrated them by means of either unspecialized hyphae ...

journal_title：Canadian journal of microbiology

authors： Tzean SS,Estey RH

更新日期：1978-07-01 00:00:00

abstract：:The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...

journal_title：Canadian journal of microbiology

authors： Ishiguro EE,Ramey WD

更新日期：1978-06-01 00:00:00

abstract：:A test is described for selective inhibition of Mycobacterium tuberculosis and M. bovis isolates in fluid medium. The method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (NAP) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other Mycobacteria. ...

journal_title：Canadian journal of microbiology

authors： Laszlo A,Eidus L

更新日期：1978-06-01 00:00:00